PURIFICATION AND CHARACTERIZATION OF PECTIN METHYLESTERASES FROM MUNG BEAN HYPOCOTYL CELL-WALLS

被引：36
作者：
BORDENAVE, M
GOLDBERG, R
机构：
[1] Ecole Normale Supérieure, Laboratoire des Biomembranes et Surfaces Cellulaires Végétales, U.A. 311, 46, rue d'Ulm
来源：
PHYTOCHEMISTRY | 1993年 / 33卷 / 05期
关键词：
VIGNA-RADIATA; FABACEAE; MUNG BEAN; PLANT CELL WALL; HYPOCOTYL; PECTIN METHYLESTERASE;
D O I：
10.1016/0031-9422(93)85011-F
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A saline eluate of Mung bean hypocotyl cell walls was submitted to carboxy-methyl Sepharose chromatography. Detection of pectin methylesterase (PME) activities after IEF of the active fractions revealed the occurrence of at least four PME isoforms. These isoforms were further purified by cation exchange chromatography, and their M(r) pH sensitivity and substrate specificity determined.
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页码：999 / 1003
页数：5
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