ISOLATION OF THE VMA-4 GENE ENCODING THE 26 KDA SUBUNIT OF THE NEUROSPORA-CRASSA VACUOLAR ATPASE

被引：27

作者：

BOWMAN, EJ ^{[1
]}

STEINHARDT, A ^{[1
]}

BOWMAN, BJ ^{[1
]}

机构：

[1] UNIV CALIF SANTA CRUZ,DEPT BIOL,SINSHEIMER LABS,SANTA CRUZ,CA 95064

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1995年 / 1237卷 / 01期

关键词：

VACUOLAR ATPASE; ATPASE; F-TYPE; VACUOLE; PROTON PUMP; GAMMA SUBUNIT; (NEUROSPORA CRASSA);

D O I：

10.1016/0005-2736(95)00108-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated the uma-4 gene, which encodes a 25 746 Dalton subunit of the vacuolar ATPase, from Neurospora crassa. The gene contains two introns and was mapped to the left arm of linkage group I. Comparison of the predicted amino acid sequence with homologous proteins from Saccharomyces cerevisiae, Manduca sexta, and Bos taurus showed only 25% sequence identity. However, computer-assisted predictions of secondary structures gave similar results for all four proteins. Analysis of the sequence and the available biochemical data indicated that the uma-4 gene product may play the same structural role in the vacuolar ATPase as does the gamma-subunit in F-type ATPases.

引用

页码：95 / 98

页数：4

共 30 条

[1]

ABRAHAMS JP, 1994, NATURE, V370, P321

[2]

ARAI H, 1988, J BIOL CHEM, V263, P8796

[3]

BOWMAN BJ, 1992, J EXP BIOL, V172, P57

[4]

BOWMAN BJ, 1989, J BIOL CHEM, V264, P15606

[5] THE VACUOLAR ATPASE OF NEUROSPORA-CRASSA [J].

BOWMAN, BJ ;

VAZQUEZLASLOP, N ;

BOWMAN, EJ .

JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 1992, 24 (04) :361-370

[6] EMPIRICAL PREDICTIONS OF PROTEIN CONFORMATION [J].

CHOU, PY ;

FASMAN, GD .

ANNUAL REVIEW OF BIOCHEMISTRY, 1978, 47 :251-276

[7] STRUCTURE AND FUNCTION OF VACUOLAR CLASS OF ATP-DRIVEN PROTON PUMPS [J].

FORGAC, M .

PHYSIOLOGICAL REVIEWS, 1989, 69 (03) :765-796

[8]

FOURY F, 1990, J BIOL CHEM, V265, P18554

[9] ANALYSIS OF ACCURACY AND IMPLICATIONS OF SIMPLE METHODS FOR PREDICTING SECONDARY STRUCTURE OF GLOBULAR PROTEINS [J].

GARNIER, J ;

OSGUTHORPE, DJ ;

ROBSON, B .

JOURNAL OF MOLECULAR BIOLOGY, 1978, 120 (01) :97-120

[10]

GOGARTEN JP, 1992, J EXP BIOL, V172, P137

← 1 2 3 →