CLONING OF HSP70 (DNAK) GENE FROM CLOSTRIDIUM-PERFRINGENS USING A GENERAL POLYMERASE CHAIN-REACTION BASED APPROACH

被引：33

作者：

GALLEY, KA ^{[1
]}

SINGH, B ^{[1
]}

GUPTA, RS ^{[1
]}

机构：

[1] MCMASTER UNIV, DEPT BIOCHEM, HAMILTON L8N 3Z5, ONTARIO, CANADA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1130卷 / 02期

基金：

英国医学研究理事会;

关键词：

HSP70-SEQUENCE; DNAK GENE; POLYMERASE CHAIN REACTION; (CLOSTRIDIUM-PERFRINGENS);

D O I：

10.1016/0167-4781(92)90529-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A general method to clone the HSP70 gene from any species employing polymerasc chain reaction and degenerate oligonucleotide primers for conserved regions of this protein family is described. Using this method, a clone containing the entire coding sequence for the HSP70 gene from Clostridium perfringens, has been isolated and sequenced. The HSP70s from C perfringens as well as other Gram-positive groups of bacteria contain a large deletion of 25 amino acids, near the N-terminal end, which is not seen in HSP70 from other sources.

引用

页码：203 / 208

页数：6

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