REGULATION OF BRANCHED-CHAIN AMINO-ACID BIOSYNTHESIS IN NEUROSPORA-CRASSA - CLONING AND CHARACTERIZATION OF THE LEU-1 AND ILV-3 GENES

被引：6

作者：

JARAI, G ^{[1
]}

YAGMAI, B ^{[1
]}

FU, YH ^{[1
]}

MARZLUF, GA ^{[1
]}

机构：

[1] OHIO STATE UNIV,DEPT BIOCHEM,COLUMBUS,OH 43210

来源：

MOLECULAR & GENERAL GENETICS | 1990年 / 224卷 / 03期

关键词：

NEUROSPORA; REGULATION; AMINO ACID SYNTHESIS;

D O I：

10.1007/BF00262432

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The genes coding for the branched-chain amino acid biosynthetic enzymes comprise an integrated regulatory system. The expression of the several structural genes coding for enzymes of the isoleucine-valine and leucine pathways is controlled in parallel by the positive-acting regulatory gene, leu-3. The leu-1 and ilv-3 genes, coding for beta-isopropyl-malate dehydrogenase and acetohydroxyacid synthase, respectively, were cloned from a cosmid library. Restriction fragment length polymorphism analysis revealed that the two cloned fragments indeed mapped to the genomic locations of the leu-1 and ilv-3 genes, respectively. Northern blot analysis demonstrated that the leu-1 gene is transcribed to give an mRNA of approximately 1.5 kb, whereas the ilv-3 transcript size is 2.6 kb. The expression of both genes appears to be regulated at the transcriptional level. One leu-3 regulatory mutant was greatly deficient in both leu-1 and ilv-3 mRNAs, whereas another leu-3 allele showed an unusual antiparallel pattern of regulation.

引用

页码：383 / 388

页数：6

共 24 条

[1] EFFECT OF ALPHA-ISOPROPYLMALATE ON THE SYNTHESIS OF RNA AND PROTEIN IN NEUROSPORA [J].