DEPENDENCE OF THYMOCYTE APOPTOSIS ON PROTEIN-KINASE-C AND PHOSPHOLIPASE A(2)

被引：29
|
作者：
SHAPOSHNIKOVA, VV
DOBROVINSKAYA, OR
EIDUS, LK
KORYSTOV, YN
机构：
[1] Laboratory of Cell Radiobiology, Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino
来源：
FEBS LETTERS | 1994年 / 348卷 / 03期
关键词：
IRRADIATION; THYMOCYTE APOPTOSIS; PROTEIN KINASE C; PHOSPHOLIPASE A(2);
D O I：
10.1016/0014-5793(94)00616-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effect of inhibitors and activators of protein kinase C and phospholipase A(2) on radiation-induced apoptosis of rat and mouse thymocytes has been studied. It is shown that the apoptosis is prevented by the protein kinase C inhibitor 1-(5-isoquinolinylsulfonyl)-2-methylpiperasine dihydrochloride and is potentiated by protein kinase C activator phorbol 12-myristate 13-acetate, calcium ionophore A23187 and concanavalin A. The protein kinase C activators initiate apoptosis in mouse but not in rat thymocytes. The inhibitor of phospholipase A(2) prevents apoptosis induced by all the factors. The results obtained indicate that both protein kinase C and phospholipase A(2) are involved in the thymocyte apoptosis.
引用
收藏
页码：317 / 319
页数：3
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