ENZYMATIC RESOLUTION OF (S)-(+)-NAPROXEN IN A CONTINUOUS REACTOR

被引：110

作者：

BATTISTEL, E ^{[1
]}

BIANCHI, D ^{[1
]}

CESTI, P ^{[1
]}

PINA, C ^{[1
]}

机构：

[1] IST G DONEGANI SPA MONTEDISON,I-28100 NOVARA,ITALY

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1991年 / 38卷 / 06期

关键词：

NAPROXEN; LIPASE; STEREOSELECTIVE HYDROLYSIS; IMMOBILIZED ENZYME; CONTINUOUS PROCESS;

D O I：

10.1002/bit.260380611

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An enzymatic method for the continuous production of (S)-(+)-2-(6-methoxy-2-naphthyl) propionic acid (Naproxen) has been developed. The process consists of a stereoselective hydrolysis of the racemic Naproxen ethoxyethyl ester catalyzed by Candida cylindracea lipase. The reaction has been carried out in a continuous-flow closed-loop column bioreactor packed with Amberlite XAD-7, a slightly polar resin on which the lipase has been immobilized by adsorption. Various immobilization conditions as well as the properties of the immobilized lipase have been studied. The performance and the productivity of the bioreactor were evaluated as a function of the critical reaction parameters such as temperature, substrate concentration, and product inhibition. By using a 500-mL column bioreactor, 1.8 kg of optically pure (S)-(+)-Naproxen were produced after 1200 h of continuous operation with a slight loss of the enzymatic activity.

引用

页码：659 / 664

页数：6

共 26 条

[1] PROTECTING EFFECT OF COMPETITIVE INHIBITORS DURING VERY INTENSE INSOLUBILIZED ENZYME-ACTIVATED SUPPORT MULTIPOINT ATTACHMENTS - TRYPSIN (AMINE)-AGAROSE (ALDEHYDE) SYSTEM [J].