EFFICIENCY OF NITRILOTRIACETATE IN THE REMOVAL OF TYPE-2 COPPER FROM LACCASE AND ASCORBATE OXIDASE

被引：10
作者：
GRAZIANI, MT
LORETI, P
MORPURGO, L
SAVINI, I
AVIGLIANO, L
机构：
[1] UNIV ROME LA SAPIENZA,DIPARTIMENTO SCI BIOCHIM,I-00185 ROME,ITALY
[2] UNIV ROMA TOR VERGATA,DIPARTIMENTO MED SPERIMENTALE & SCI BIOCHIM,I-00173 ROME,ITALY
[3] UNIV LAQUILA,DIPARTIMENTO SCI & TECNOL BIOMED & BIOMETRIA,I-67100 LAQUILA,ITALY
来源：
INORGANICA CHIMICA ACTA | 1990年 / 173卷 / 02期
关键词：
D O I：
10.1016/S0020-1693(00)80222-4
中图分类号：
O61 [无机化学];
学科分类号：
070301 ; 081704 ;
摘要：
The powerful chelating agent nitrilotriacetate was found to be quite efficient in the removal of type 2 Cu, under reducing conditions, from the blue oxidases ascorbate oxidase and Rhus vernicifera laccase. While the effect of this substance on ascorbate oxidase was comparable to that of other reagents, as for instance EDTA, the effect on laccase was substantially larger than that of any other previously tested chelator, in particular EDTA which is by itself ineffective. This result confirms that the size of the chelator strongly affects its reactivity with laccase type 2 Cu, suggesting that the latter ion is more deeply buried in the protein matrix than the copper of ascorbate oxidase. © 1990.
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页码：261 / 264
页数：4
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