Germplasm conservation of Clitoria ternatea L. through storage of encapsulated shoot tips and maintaining nodal segments under slow growth conditions

被引：0

作者：

Jyoti, Dixit ^{[1
]}

Jayanti, Singh ^{[1
]}

Nath, Tiwari Kavindra ^{[1
]}

机构：

[1] Banaras Hindu Univ, MMV, Dept Bot, Varanasi 21005, Uttar Pradesh, India

来源：

RESEARCH JOURNAL OF BIOTECHNOLOGY | 2018年 / 13卷 / 03期

关键词：

Clitoria ternatea; conversion; encapsulated shoot tips; osmoticum;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The presence of 3% sodium alginate + 100 mu M calcium chloride was found most suitable for Clitoria ternatea synthetic seed production and subsequent conversion of encapsulated shoot tips into plantlet. MS medium used for encapsulation of shoot tips showed highest conversion frequency (90 +/- 7.0%) on MS medium supplemented with 1.0mg/l BAP. Encapsulated shoot tips stored for 75 days at 4 degrees C showed maximum conversion frequency (52 +/- 3.2%) on MS + 1.0mg/l BAP + 0.1mg/l IBA. Nodal segmented stored on 1/2 MS medium supplemented with 4% sorbitol for 12 months showed highest conversion frequency (65%) and maximum number of shoots per explants on MS medium containing 0.5mg/l BAP. Elongated shoots showed best rooting on MS + 0.2 mg/l IBA. Plants derived from short term stored synthetic seeds and nodal segment showed normal flowering and growth.

引用

页码：54 / 62

页数：9