CORRELATION OF ELECTROPHORETIC MOBILITIES FROM CAPILLARY ELECTROPHORESIS WITH PHYSICOCHEMICAL PROPERTIES OF PROTEINS AND PEPTIDES

被引：231

作者：

RICKARD, EC ^{[1
]}

STROHL, MM ^{[1
]}

NIELSEN, RG ^{[1
]}

机构：

[1] HYBRITECH INC,MFG & TECH SUPPORT,SAN DIEGO,CA 92126

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 197卷 / 01期

关键词：

D O I：

10.1016/0003-2697(91)90379-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Excellent correlation was observed for the electrophoretic mobilities measured by capillary zone electrophoresis versus q MW 2 3, where q is the calculated charge and MW is the molecular weight. Mobilities of a set of 33 diverse peptides from enzymatic digests and 10 intact proteins were measured for separations at pH 2.35, 8.0, and 8.15 with constant ionic strength, temperature, and viscosity. The correlation suggests that the frictional drag is proportional to the surface area of a sphere that has a volume proportional to the MW. The correlation of electrophoretic mobility with physicochemical properties will facilitate the elucidation of optimum separation strategies for protein and peptide mixtures. © 1991.

引用

页码：197 / 207

页数：11

共 34 条

[21]

NIELSEN RG, 1990, ACS S SERIES, V434, pCH2

[22]

NYBERG F, 1988, ELECTROPHORESIS 88, P141

[23] ELECTROPHORETIC MOBILITIES OF PEPTIDES ON PAPER AND THEIR USE IN DETERMINATION OF AMIDE GROUPS [J].