USE OF COIL PLANET CENTRIFUGE IN THE ISOLATION OF ANTIBIOTICS

In the course of isolating antibiotics from actinomycete fermentations, we have found the Ito Multi-Layer Coil Planet Centrifuge (CPC) can effect separations of such quality on crude fermentation extracts, that frequently, meaningful spectral data may be obtained on the separated bioactive component from a single counter-current chromatography. This paper outlines the use of CPC counter-current chromatography. This paper outlines the use of CPC counter-current distribution in the isolation-separation of siderochelin A, efrotomycin, pentalenolactone, Bu 2313 B, and the closely related congeners, tirandamycins A and B. The discussion includes general methods for solvent system selection and sample preparation. Sepectral data on the isolated compounds are presented as evidence for the quality of separations effected.