PRESSURIZED GRADIENT ELECTRO-HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：157

作者：

BEHNKE, B ^{[1
]}

BAYER, E ^{[1
]}

机构：

[1] UNIV TUBINGEN,INST ORGAN CHEM,D-72076 TUBINGEN,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 680卷 / 01期

关键词：

D O I：

10.1016/0021-9673(94)80056-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A microbore liquid chromatographic system was developed for gradient elution using capillary columns with inner diameters of 50 and 100 mu m. In addition, voltage gradients up to 30 000 V can be applied across the length of the column. The dramatic improvement of reversed-phase separations of charged analytes is demonstrated by a separation of detritylated oligonucleotides on 5 mu m C-18 reversed-phase silica gel. Several examples are given, illustrating the influence of applied voltage gradients up to 400 V/cm upon the separation in both isocratic and gradient elution modes.

引用

页码：93 / 98

页数：6