PRESSURIZED GRADIENT ELECTRO-HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引:157
作者
BEHNKE, B [1 ]
BAYER, E [1 ]
机构
[1] UNIV TUBINGEN,INST ORGAN CHEM,D-72076 TUBINGEN,GERMANY
关键词
D O I
10.1016/0021-9673(94)80056-1
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A microbore liquid chromatographic system was developed for gradient elution using capillary columns with inner diameters of 50 and 100 mu m. In addition, voltage gradients up to 30 000 V can be applied across the length of the column. The dramatic improvement of reversed-phase separations of charged analytes is demonstrated by a separation of detritylated oligonucleotides on 5 mu m C-18 reversed-phase silica gel. Several examples are given, illustrating the influence of applied voltage gradients up to 400 V/cm upon the separation in both isocratic and gradient elution modes.
引用
收藏
页码:93 / 98
页数:6
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