CHARACTERIZATION OF A MEMBRANE FRAGMENT OF RESPIRATORY-CHAIN COMPLEX-I FROM NEUROSPORA-CRASSA - INSIGHTS ON THE TOPOLOGY OF THE UBIQUINONE-BINDING SITE

被引：9

作者：

AZEVEDO, JE

VIDEIRA, A

机构：

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1994年 / 26卷 / 04期

关键词：

COMPLEX I; NEUROSPORA CRASSA; UBIQUINONE-BINDING SITE;

D O I：

10.1016/0020-711X(94)90007-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. A membrane fragment of complex I from the fungus Neurospora crassa was isolated by immunoprecipitation from alkaline-extracted mitochondrial membranes. 2. Analysis of the polypeptide composition of this hydrophobic domain of complex I has brought insights on the topology of two subunits of the enzyme, namely the 20.8 and 9.3 kDa components. 3. Our results indicate that the ubiquinone-binding site of complex I resides in the interface of the peripheral and membrane arms of the enzymes. The significance of these findings are discussed.

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页码：505 / 510

页数：6

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