A VALIDATED HPTLC METHOD FOR DETERMINATION OF TERBINAFINE HYDROCHLORIDE IN PHARMACEUTICAL SOLID DOSAGE FORM

An HPTLC method for estimation of Terbinafine hydrochloride in its tablet formulation has been developed. It employs aluminium backed silica gel 60 F254 TLC plates,(100x50 mm, thickness of layer 0.2 mm), prewashed with methanol and dried at room temperature) and mobile phase comprising of Acetonitrile: 1, 4 dioxan: Hexane: Acetic acid (1:1:8:0.1) (v/v/v/v). The developing solvent was run upto 70 mm in Camag chamber. Densitometic scanning was then performed with Camag TLC scanner-3 at lambda max 282 nm. The R-f value was found to be 0.45. The linearity and range for Terbinafine Hydrochloride was found to be 500-4500ng/spot. The method was validated for precision, accuracy and sensitivity.