TRANSFORMATION OF ASPERGILLUS-NIDULANS BY MICROPROJECTILE BOMBARDMENT ON INTACT CONIDIA

被引：0

作者：

FUNGARO, MHP

RECH, E

MUHLEN, GS

VAINSTEIN, MH

PASCON, RC

DEQUEIROZ, MV

PIZZIRANIKLEINER, AA

DEAZEVEDO, JL

机构：

[1] CENARGEN EMBRAPA,BR-70849970 BRASILIA,DF,BRAZIL

[2] UNIV BRASILIA,DEPT BIOL CELULAR,BR-70910900 BRASILIA,DF,BRAZIL

来源：

FEMS MICROBIOLOGY LETTERS | 1995年 / 125卷 / 2-3期

关键词：

ASPERGILLUS NIDULANS; FUNGAL TRANSFORMATION; BIOLISTIC GENE TRANSFER; CONIDIAL TRANSFORMATION;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This paper describes transformation of intact conidia of Aspergillus nidulans, auxotrophic for arginine, by using the biolistic process. The plasmid employed was pFB39, carrying the argB gene. The transformation frequency obtained was 81 transformants/mu g of DNA. Classical genetics and molecular analysis were conducted to analyse transformants and to determine in which chromosome integration took place.

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页码：293 / 297

页数：5

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