SYNTHESIS AND CHARACTERIZATION OF A POLYMERIC FLUOROCARBON-DIAMINE REVERSED-PHASE WEAK ANION-EXCHANGE SILICA HPLC COLUMN PACKING

Polymeric fluorocarbon-diamine silica column packings were synthesized by first reacting a copolymer of chlorotrifluoroethylene and vinylidene fluoride (Kel-F 800) with piperazine and then reacting this product with aminopropyl silica. This mixed mode reversed phase-weak anion exchange HPLC column and a hydrocarbon (C-8) weak anion exchange silica HPLC column were compared for the separation of aromatic organic acids. Although the fluorocarbon column was generally less retentive than the hydrocarbon one, good resolution and fast analysis of simple mixtures was still possible. Nucleotides were retained longer on the fluorocarbon column. Good alkaline pH stability of the polymeric fluorocarbon-diamine silica column packing was exhibited.