ENRICHMENT AND CHARACTERIZATION OF DENDRITIC CELLS FROM HUMAN BRONCHOALVEOLAR LAVAGES

被引:0
|
作者
HAVENITH, CEG
VANHAARST, JMW
BREEDIJK, AJ
BETJES, MGH
HOOGSTEDEN, HC
BEELEN, RHJ
HOEFSMIT, ECM
机构
[1] FREE UNIV AMSTERDAM,FAC MED,DEPT CELL BIOL,DIV ELECTRONMICROSCOPY,1081 BT AMSTERDAM,NETHERLANDS
[2] ERASMUS UNIV ROTTERDAM,DEPT IMMUNOL,3000 DR ROTTERDAM,NETHERLANDS
来源
CLINICAL AND EXPERIMENTAL IMMUNOLOGY | 1994年 / 96卷 / 02期
关键词
PULMONARY IMMUNE RESPONSES; MIXED LEUKOCYTE REACTION; DENDRITIC CELLS; BRONCHOALVEOLAR LAVAGES;
D O I
暂无
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
In the present study about 0.3% to 1.6% of human bronchoalveolar lavage (BAL) cells were identified as typical dendritic cells (DC), having an irregular outline, lobulated nucleus, and clear distinguishable acid phosphatase activity or EBM11 (anti-CD68) reactivity in a spot near the nucleus. After DC enrichment, using transient adherence to plastic, FcR-panning, and a density metrizamide gradient, a population containing 7-8% typical DC was obtained. This DC-enriched low density fraction, containing the highest percentages of DC, very strongly induced T cell proliferation in an allogeneic mixed leucocyte reaction (MLR), which was significantly higher than that induced by other partly (un)fractionated BAL cells. These data indicate that DC seem to be the major accessory cells in the BAL fluid, and therefore may be important in the regulation of T cell immune responses in the lung.
引用
收藏
页码:339 / 343
页数:5
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