METABOLISM OF (+)-ABSCISIC ACID TO (+)-7'-HYDROXYABSCISIC ACID BY BROMEGRASS CELL-CULTURES

被引：43

作者：

HAMPSON, CR

REANEY, MJT

ABRAMS, GD

ABRAMS, SR

GUSTA, LV

机构：

[1] UNIV SASKATCHEWAN,CTR CROP DEV,SASKATOON S7N 0W0,SASKATCHEWAN,CANADA

[2] PRAIRIE MALT LTD,BIGGAR S0K 0M0,SASKATCHEWAN,CANADA

[3] NATL RES COUNCIL CANADA,INST PLANT BIOTECHNOL,SASKATOON S7N 0W9,SASKATCHEWAN,CANADA

来源：

PHYTOCHEMISTRY | 1992年 / 31卷 / 08期

关键词：

BROMUS-INERMIS; GRAMINEAE; CELL CULTURES; METABOLISM; ABSCISIC ACID; (+)-7'-HYDROXYABSCISIC ACID; (-)-7'-HYDROXYABSCISIC ACID; CHIRAL HPLC;

D O I：

10.1016/0031-9422(92)83603-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

7'-Hydroxyabscisic acid was isolated from the medium of smooth bromegrass (Bromus inermis Leyss.) cell suspension cultures supplied with either (+/-)- or natural (+)-abscisic acid, and the identity of the metabolite confirmed by H-1 NMR. Analysis of the methyl esters of the metabolite by chiral HPLC showed only the (+) enantiomer of 7'-hydroxyabscisic acid to be present in the medium of cultures fed (+)-abscisic acid. In cultures treated with (+/-)-abscisic acid, the (-) enantiomer of the metabolite is formed from the (-)-abscisic acid component of the racemic mixture supplied.

引用

页码：2645 / 2648

页数：4

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