AN ORIGIN OF DNA-REPLICATION AND A TRANSCRIPTION SILENCER REQUIRE A COMMON ELEMENT

被引：130
作者：
RIVIER, DH
RINE, J
机构：
[1] Division of Genetics, Department of Molecular and Cell Biology, University of California, Berkeley
来源：
SCIENCE | 1992年 / 256卷 / 5057期
关键词：
D O I：
10.1126/science.1585179
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A eukaryotic chromosomal origin of replication was identified in the yeast Saccharomyces cerevisiae. By several criteria, including map position, deletion analysis, and a synthetic form of saturation mutagenesis, the origin co-localized with the HMR-E silencer, which is a DNA element that represses transcription of the adjacent genes. A specific site within the silencer was required for both initiation of chromosomal replication and for repression of transcription. This analysis directly demonstrates that initiation of eukaryotic chromosomal replication is dependent on specific sequence elements and that a particular element can act in both initiation of chromosomal replication and regulation of transcription.
引用
收藏
页码：659 / 663
页数：5
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