A SENSITIVE AND SPECIFIC ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR METHIONINE ENKEPHALIN SULFOXIDE

被引：1
作者：
TIONG, GKL
OLLEY, JE
机构：
[1] Department of Pharmacology, Monash University, Clayton, Vic.
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 131卷 / 01期
关键词：
ELISA; Methionine enkephalin; Methionine enkephalin sulfoxide;
D O I：
10.1016/0022-1759(90)90233-L
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An enzyme-linked immunosorbent assay (ELISA) for the sulphoxide derivative of the opioid peptide methionine enkephalin (MOE) was developed and found to be sensitive and highly specific. Methionine enkephalin itself showed only low cross-reactivity (1.8%) in the assay while leucine enkephalin and the endorphins showed cross-reactivities no higher than 0.6%. Given the ease of application, low cost and stable nature of the label, this technique is a useful alternative to existing radioimmunoassays for the peptide. © 1990.
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页码：65 / 69
页数：5
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