ELISA METHOD FOR DETECTION OF INFLUENZA-A INFECTION IN SWINE

被引：30

作者：

LEE, BW

BEY, RF

BAARSCH, MJ

SIMONSON, RR

机构：

[1] Department of Veterinary PathoBiology, School of Veterinary Medicine, University of Minnesota, St. Paul

[2] Oxford Laboratories, Number One Bio Drive, Worthington, MN 56187

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 1993年 / 5卷 / 04期

关键词：

D O I：

10.1177/104063879300500402

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

An antigen-capture enzyme-linked immunosorbent assay (ELISA) was developed to monitor virus shedding associated with experimental infection with a field strain of swine influenza in pigs. The assay consisted of a monoclonal anti-nucleoprotein capture antibody and a biotinylated rabbit anti-influenza A (H1N1) sandwich antibody. The antigen-capture system was capable of detecting as little as 1 ng/ml purified vints. The ELISA system surpassed egg cultivation procedures in the detection of low levels of shedding virus. Egg cultivation procedures indicated that most viral shedding had ceased by day 10 postinfection. In contrast, antigen-capture ELISA still showed an ongoing presence of viral antigen. A virus-capture ELISA, using this capture-sandwich antibody system, is equivalent in sensitivity to conventional egg inoculation procedures for the detection of the early phases of virus shedding. The automative potential of an ELISA-based system coupled with a substantially reduced assay time requirement give this virus-capture ELISA a distinct advantage over other cell culture or egg-based diagnostic techniques.

引用

页码：510 / 515

页数：6

共 28 条

[1]

Beard C.W., Influenza, A laboratory manual for the isolation and identification of avian pathogens, pp. 110-113, (1989)

[2]

Blaskovic D., Jamrichova O., Rathova V., Experimental infection of weanling pigs with A/swine influenza virus, Bull WHO, 42, pp. 767-770, (1970)

[3]

Bressoud A., Witcomb J., Pourzand C., Rapid detection of influenza virus H 1 by the polymerase chain reaction, Biochem Biophys Res Comm, 167, pp. 425-430, (1990)

[4]

Bucher D.J., Mikhail A., Popple S., Rapid detection of type A influenza with monoclonal antibodies to the M protein (M 1) by enzyme-linked immunosorbant assay and time resolved fluoroimmunoassay, J Clin Microbiol, 29, pp. 2484-2488, (1991)

[5]

Chambers T.M., Hinshaw V.S., Kawaoka Y., Influenza viral infection of swine in the United States 1988—1989, Arch Virol, 116, pp. 261-265, (1991)

[6]

Chomel J.J., Pardon D., Thouvenot D., Comparison between three rapid methods for direct diagnosis of influenza and the conventional isolation procedure, Biologicals, 19, pp. 287-292, (1991)

[7]

Chomel J.J., Thouvenot D., Onno M., Rapid diagnosis of influenza infection of NP antigen using an immunocapture ELISA test, J Virol Methods, 25, pp. 81-92, (1989)

[8]

Cook R.F., Sinclair R., Mumford J.A., Detection of influenza nucleoprotein antigen in nasal secretions from horses infected with A/equine influenza (H3N8) viruses, J Virol Methods, 20, pp. 1-12, (1988)

[9]

Coonrod J.D., Karathanasis P., Betts R.F., Donofrio J.C., Enzyme-linked immunosorbant assay of core antigens for clinical diagnosis of influenza, J Med Virol, 25, pp. 399-409, (1988)

[10]

Easterday B.C., Immunologic considerations in swine influenza, J Am Vet Med Assoc, 160, pp. 645-648, (1972)

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