FORMATION OF INULO-OLIGOSACCHARIDES BY BETA-FRUCTOFURANOSIDASE FROM ASPERGILLUS-ORYZAE

Inulo-oligosaecharides(I) production, by use of immobilized β-fructofuranosidase from Aspergillus Oryzae was investigated. Chito-pearl-BCW-4010 was the best carrier for immobilization of the enzyme. The optimum temperature was 25°C and the optimum pH was 6. 8 for production of (I) from sucrose and fructose. When the molar fraction of sucrose was increased for sake of forming of enzyme-fructose complex, sucrose acted as an acceptor sugar to form fructooligosaccharides. This dose not contribute to formation of (I). A disubtituted sucrose derivative retaining (Fruf2βa 1 Glcp) bond reacted with immobilized enzyme to form the complex. This complex reacted with fructose to form the disubtituted inulobiose which was converted to inulobiose in good yield. © 1990, The Chemical Society of Japan. All rights reserved.