PREPARATION OF PLANT-SAMPLES FOR HIGH-PRECISION NITROGEN ISOTOPE RATIO ANALYSIS

被引：31
作者：
UNKOVICH, M
PATE, JS
SANFORD, P
机构：
[1] UNIV WESTERN AUSTRALIA, COOPERAT RES CTR LEGUMES MEDITERRANEAN AGR, NEDLANDS, WA 6009, AUSTRALIA
[2] WESTERN AUSTRALIA DEPT AGR, ALBANY 6330, AUSTRALIA
来源：
COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS | 1993年 / 24卷 / 15-16期
关键词：
D O I：
10.1080/00103629309368939
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Methodologies for preparation of plant samples for high precision nitrogen isotope ratio analysis are described, including detailed attention to Kjeldhal digestion, steam distillation, concentration of distillates, and generation of nitrogen gas (N2) from ammonium sulfate [(NH4)2SO4]. Apparatus developed in the authors laboratory is illustrated. Using a systematic approach to the assessment of errors associated with each of the steps in preparation and analysis procedures, the methods outlined in this paper are shown capable of determining deltaN-15 in plant material with a precision of -+/0.2 parts per thousand.
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页码：2093 / 2106
页数：14
相关论文
共 20 条
[11]   COMPARISON OF KJELDAHL AND COMBUSTION METHODS FOR MEASUREMENT OF NITROGEN ISOTOPE RATIOS IN ORGANIC-MATTER [J].
MINAGAWA, M ;
WINTER, DA ;
KAPLAN, IR .
ANALYTICAL CHEMISTRY, 1984, 56 (11) :1859-1861
[12]   COMPARISON OF PROCEDURES FOR REDUCING CROSS-CONTAMINATION DURING STEAM DISTILLATIONS IN N-15 TRACER RESEARCH [J].
MULVANEY, RL .
SOIL SCIENCE SOCIETY OF AMERICA JOURNAL, 1986, 50 (01) :92-96
[13]  
PEOPLES NB, 1991, STABLE ISOTOPES PLAN, P117
[14]   APPARATUS FOR PREPARING NITROGEN FROM AMMONIUM-CHLORIDE FOR N-15 DETERMINATIONS [J].
PORTER, LK ;
ODEEN, WA .
ANALYTICAL CHEMISTRY, 1977, 49 (03) :514-516
[15]   THE MEASUREMENT OF N-15 IN SOIL AND PLANT-MATERIAL [J].
PRUDEN, G ;
POWLSON, DS ;
JENKINSON, DS .
FERTILIZER RESEARCH, 1985, 6 (03) :205-218
[16]   A COMPARISON OF CROSS-CONTAMINATION IN DISTILLATION UNITS USED IN TOTAL NITROGEN AND N-15 ANALYSES [J].
REEDER, JD ;
ODEEN, WA ;
PORTER, LK ;
LOBER, RW .
SOIL SCIENCE SOCIETY OF AMERICA JOURNAL, 1980, 44 (06) :1262-1267
[17]   ERRORS IN CONCENTRATING AMMONIUM SAMPLES PRIOR TO ISOTOPE-RATIO ANALYSIS [J].
REEDER, JD .
SOIL SCIENCE SOCIETY OF AMERICA JOURNAL, 1984, 48 (03) :695-698
[18]  
RITTENBERG D, 1948, PREPARATION MEASUREM, P31
[19]   N-2-FIXATION IN FIELD SETTINGS - ESTIMATIONS BASED ON NATURAL N-15 ABUNDANCE [J].
SHEARER, G ;
KOHL, DH .
AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY, 1986, 13 (06) :699-756
[20]   VARIATION IN NATURAL ABUNDANCE OF N-15 AMONG PLANT-PARTS AND IN N-15-N-14 FRACTIONATION DURING N-2 FIXATION IN THE LEGUME-RHIZOBIA SYMBIOTIC SYSTEM [J].
YONEYAMA, T ;
FUJITA, K ;
YOSHIDA, T ;
MATSUMOTO, T ;
KAMBAYASHI, I ;
YAZAKI, J .
PLANT AND CELL PHYSIOLOGY, 1986, 27 (05) :791-799
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