A MULTIUBIQUITIN CHAIN IS CONFINED TO SPECIFIC LYSINE IN A TARGETED SHORT-LIVED PROTEIN

被引：1226
作者：
CHAU, V
TOBIAS, JW
BACHMAIR, A
MARRIOTT, D
ECKER, DJ
GONDA, DK
VARSHAVSKY, A
机构：
[1] MIT,DEPT BIOL,CAMBRIDGE,MA 02139
[2] SK&F LABS,DEPT MOLEC PHARMACOL,PHILADELPHIA,PA 19101
来源：
SCIENCE | 1989年 / 243卷 / 4898期
关键词：
D O I：
10.1126/science.2538923
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
引用
收藏
页码：1576 / 1583
页数：8
相关论文
共 39 条
[1]   INTERNAL AMINO-ACID SEQUENCE-ANALYSIS OF PROTEINS SEPARATED BY ONE-DIMENSIONAL OR TWO-DIMENSIONAL GEL-ELECTROPHORESIS AFTER INSITU PROTEASE DIGESTION ON NITROCELLULOSE [J].
AEBERSOLD, RH ;
LEAVITT, J ;
SAAVEDRA, RA ;
HOOD, LE ;
KENT, SBH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (20) :6970-6974
[2]  
Asubel F.M., 1987, CURRENT PROTOCOLS MO
[3]   THE DEGRADATION SIGNAL IN A SHORT-LIVED PROTEIN [J].
BACHMAIR, A ;
VARSHAVSKY, A .
CELL, 1989, 56 (06) :1019-1032
[4]   INVIVO HALF-LIFE OF A PROTEIN IS A FUNCTION OF ITS AMINO-TERMINAL RESIDUE [J].
BACHMAIR, A ;
FINLEY, D ;
VARSHAVSKY, A .
SCIENCE, 1986, 234 (4773) :179-186
[5]   ARTHRIN, A MYOFIBRILLAR PROTEIN OF INSECT FLIGHT-MUSCLE, IS AN ACTIN UBIQUITIN CONJUGATE [J].
BALL, E ;
KARLIK, CC ;
BEALL, CJ ;
SAVILLE, DL ;
SPARROW, JC ;
BULLARD, B ;
FYRBERG, EA .
CELL, 1987, 51 (02) :221-228
[6]  
Bornstein P, 1977, Methods Enzymol, V47, P132
[7]  
CHAU V, UNPUB
[8]   UBIQUITIN DEPENDENCE OF SELECTIVE PROTEIN-DEGRADATION DEMONSTRATED IN THE MAMMALIAN-CELL CYCLE MUTANT TS85 [J].
CIECHANOVER, A ;
FINLEY, D ;
VARSHAVSKY, A .
CELL, 1984, 37 (01) :57-66
[9]   TRYPTIC PEPTIDE-MAPPING OF UBIQUITIN AND DERIVATIVES USING REVERSE-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
COX, MJ ;
SHAPIRA, R ;
WILKINSON, KD .
ANALYTICAL BIOCHEMISTRY, 1986, 154 (01) :345-352
[10]  
ECKER DJ, 1987, J BIOL CHEM, V262, P14213
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