SOLID-PHASE EXTRACTION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF DOXOPHYLLINE IN PLASMA

被引：15

作者：

LAGANA, A ^{[1
]}

BIZZARRI, M ^{[1
]}

MARINO, A ^{[1
]}

MANCINI, M ^{[1
]}

机构：

[1] UNIV ROME LA SAPIENZA,DEPT CARDIAC SURG,I-00185 ROME,ITALY

来源：

BIOMEDICAL CHROMATOGRAPHY | 1990年 / 4卷 / 05期

关键词：

D O I：

10.1002/bmc.1130040507

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive and selective high performance liquid chromatographic doxophylline assay with ultraviolet detection has been developed for plasma samples. The drug is isolated from biological samples with a reversed phase C18 disposable extraction column. Plasma standard curves are linear for concentrations of doxophylline from 0.03 to 10 mg/L. At the therapeutic range concentrations, the recoveries are better than 96.9%. The coefficients of variation for the procedure are 4.1% and 2.7% for the concentrations 0.03 mg/L and 10 mg/L, respectively. By this method, pharmacokinetic profiles are obtained for six adult volunteers. Copyright © 1990 John Wiley & Sons, Ltd.

引用

页码：205 / 207

页数：3