ROLE OF PROTEIN-KINASE-C IN CHOLINERGIC STIMULATION OF LACRIMAL GLAND PROTEIN SECRETION

被引：26

作者：

ZOUKHRI, D ^{[1
]}

HODGES, RR ^{[1
]}

DICKER, DM ^{[1
]}

DARTT, DA ^{[1
]}

机构：

[1] HARVARD UNIV,SCH MED,BOSTON,MA 02114

来源：

FEBS LETTERS | 1994年 / 351卷 / 01期

关键词：

CARBACHOL; EXOCRINE GLAND; PROTEIN KINASE C; PHORBOL ESTER; SIGNAL TRANSDUCTION; EXOCYTOSIS;

D O I：

10.1016/0014-5793(94)00824-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The purpose of this study was to determine the role of protein kinase C (PKC) isozymes in carbachol-induced protein secretion in the lacrimal gland. Three isoforms of PKC are present in rat lacrimal gland: PKC-alpha, -delta and -epsilon. Carbachol translocated PKC-epsilon during 5 s incubation. Pretreatment with PdBu for 0 to 4 h down-regulated PKC-alpha by 31% at 20 min, PKC-epsilon by 36% at 2 h, and PKC-delta by 37% at 4 h. A 2 h phorbol ester treatment inhibited carbachol-induced secretion completely at 1 min and partially at 5, and 20 min, but did not alter the carbachol-induced increase in the intracellular [Ca2+]. We conclude that PKC-alpha. and -epsilon, but not PKC-delta, are implicated in cholinergic agonist-induced protein secretion in rat lacrimal gland.

引用

页码：67 / 72

页数：6

共 25 条

[1]

BERRIDGE MJ, 1987, ANNU REV BIOCHEM, V56, P159, DOI 10.1146/annurev.bi.56.070187.001111

[2] VASOACTIVE INTESTINAL POLYPEPTIDE STIMULATION OF PROTEIN SECRETION FROM RAT LACRIMAL GLAND ACINI [J].