FUNCTIONAL-PROPERTIES OF RAT-BRAIN SODIUM-CHANNELS EXPRESSED IN A SOMATIC-CELL LINE

被引：100

作者：

SCHEUER, T ^{[1
]}

AULD, VJ ^{[1
]}

BOYD, S ^{[1
]}

OFFORD, J ^{[1
]}

DUNN, R ^{[1
]}

CATTERALL, WA ^{[1
]}

机构：

[1] UNIV TORONTO,DEPT MED GENET,TORONTO M5S 1A1,ONTARIO,CANADA

来源：

SCIENCE | 1990年 / 247卷 / 4944期

关键词：

D O I：

10.1126/science.2154850

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Transfection of Chinese hamster ovary cells with complementary DNA encoding the RIIA sodium channel a subunit from rat brain led to expression of functional sodium channels with the rapid, voltage-dependent activation and inactivation characteristic of sodium channels in brain neurons. The sodium currents mediated by these transfected channels were inhibited by tetrodotoxin, persistently activated by veratridine, and prolonged by Leiurus α-scorpion toxin, indicating that neurotoxin receptor sites 1 through 3 were present in functional form. The RIIA sodium channel a subunit cDNA alone is sufficient for stable expression offunctional sodium channels with the expected kinetic and pharmacological properties in mammalian somatic cells.

引用

页码：854 / 858

页数：5

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