ADENOVIRUS STRUCTURE AS REVEALED BY X-RAY CRYSTALLOGRAPHY, ELECTRON-MICROSCOPY, AND DIFFERENCE IMAGING

被引：5

作者：

STEWART, PL

BURNETT, RM

机构：

[1] The Wistar Institute, Philadelphia, PA, 19104

来源：

JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS SHORT NOTES & REVIEW PAPERS | 1993年 / 32卷 / 3B期

关键词：

ADENOVIRUS; X-RAY DIFFRACTION; ELECTRON MICROSCOPY; DIFFERENCE IMAGING; 3-DIMENSIONAL STRUCTURE;

D O I：

10.1143/JJAP.32.1342

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

The three-dimensional structure of human type 2 adenovirus was studied by combining X-ray crystallography and electron microscopy in a novel way. The 2.9 angstrom crystal structure of the major capsid protein, hexon, was positioned into a three-dimensional image reconstruction of the intact virus that was derived from cryo-electron micrographs. A three-dimensional difference map was generated by subtracting 240 copies of the crystallographic hexon from the density of the intact virus. This map revealed several minor structural proteins acting as ''cement'' to stabilize the assembly. The current state of structural knowledge concerning the location of the polypeptide components and the viral DNA is presented.

引用

页码：1342 / 1347

页数：6

共 33 条

[1] CRYO-ELECTRON MICROSCOPY OF VIRUSES [J].