GLOBAL ANALYSIS OF WAVELENGTH-RESOLVED FLUORESCENCE DECAY IN SARCOPLASMIC-RETICULUM CALCIUM-ATPASE

被引:5
作者
FERREIRA, ST
VERJOVSKIALMEIDA, S
机构
[1] Departamento de Bioquímica, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro
关键词
D O I
10.1016/0022-2313(91)90153-M
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
The intrinsic fluorescence decay of sarcoplasmic reticulum Ca2+-ATPase was measured in the frequency-domain at 10 nm intervals across the emission spectrum. Data were globally analysed in terms of a unique set of lifetime values, allowing for changes in fractional intensities of each lifetime across the emission. The wavelength-resolved decay was optimally described by a Lorentzian distribution of lifetimes plus two exponential terms. Analysis of chi-2 surfaces associated with uncertainties in fitting parameters revealed that the recovered parameters were well-defined despite the complexity of the decay. Thus, global analysis enables resolution of ATPase fluorescence into decay-associated spectral components, which should permit investigation of ligand-induced changes in each individual component.
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收藏
页码:430 / 434
页数:5
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