PHENYLALANINE AS SUBSTRATE FOR TYROSINE-HYDROXYLASE IN BOVINE ADRENAL CHROMAFFIN CELLS

被引：34

作者：

FUKAMI, MH

HAAVIK, J

FLATMARK, T

机构：

[1] Department of Biochemistry, University of Bergen

来源：

BIOCHEMICAL JOURNAL | 1990年 / 268卷 / 02期

关键词：

D O I：

10.1042/bj2680525

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Incubation of bovine chromaffin cells with L-[14C]phenylalanine resulted in label accumulation in catecholamines at about 30% of the rate seen with L-tyrosine as precursor. Studies with purified tyrosine hydroxylase (EC 1.14.16.2) showed that the enzyme catalysed the hydroxylation of L-phenylalanine first to L-p-tyrosine and then to 3,4-dihydroxyphenylalanine (DOPA). No evidence for a significant involvement of an L-m-tyrosine intermediate in DOPA formation was found.

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页码：525 / 528

页数：4

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