PREPARATION OF [C-14] URIDINE 5'-DIPHOSPHATE AND [C-14] GUANOSINE 5'-DIPHOSPHATE

被引:5
|
作者
CORY, JG
BACON, PE
机构
来源
PREPARATIVE BIOCHEMISTRY | 1984年 / 14卷 / 03期
关键词
D O I
10.1080/10826068408070631
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Procedures were developed for the routine enzymatic synthesis of [14C]UDP and [14C]GDP from commercially available enzymes and [14C]UMP and [14C]GMP. Using high pressure liquid chromatography, the products are recovered in high yield (60-80%) and with high purity. The [14C]UDP and [14C]GDP are utilized as substrates for ribonucleotide reductase.
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页码:231 / 237
页数:7
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