APPROACHES TO OPTIMIZATION OF PRECISION IN CAPILLARY ELECTROPHORESIS

A study has been performed to obtain insight into the relative importance of critical factors affecting the repeatability of hydrodynamic injections in CE. Precision was measured for repeated analysis of a test mixture containing two acidic compounds. The use of an internal standard was clearly shown to improve precision especially when peak area precision was poor. It is suggested that precision is maximised by employing a combination of a constant temperature, an appropriate electrolyte system, an internal standard, long injection times, and high sample concentrations. Other factors are discussed, but are classified as having only a minor impact.