CHARACTERIZATION OF A MONOCLONAL-ANTIBODY DIRECTED AGAINST THE PHOSPHOTYROSINE KINASE P56LCK, IN HUMAN T-CELLS

被引：10
|
作者：
ANSOTEGUI, IJ
CHOW, SC
JEDDITEHRANI, M
MELOCHE, S
PAWSON, A
SEKALY, RP
MAK, TW
WIGZELL, H
机构：
[1] ONTARIO CANC INST,TORONTO M4X 1K9,ONTARIO,CANADA
[2] INST RECH CLIN MONTREAL,IMMUNOL LAB,MONTREAL H2W 1R7,QUEBEC,CANADA
[3] SAMUEL LUNESFELD RES INST,TORONTO,ONTARIO,CANADA
来源：
SCANDINAVIAN JOURNAL OF IMMUNOLOGY | 1991年 / 33卷 / 04期
关键词：
D O I：
10.1111/j.1365-3083.1991.tb01784.x
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
A monoclonal antibody (anti-p56lck) was generated against a fusion protein containing the residues 145-509 of the human p56lck, a lymphocyte-specific membrane-associated protein tyrosine kinase. The involvement of this enzyme in T-cell transmembrane signalling seems to be an early and crucial event during T-cell receptor-mediated activation. We have produced a monoclonal antibody which recognizes p56lck in free form and when associated with CD4. It functions in western blot analysis and is capable of selectively blocking auto-phosphorylation of this kinase. This monoclonal antibody should be useful for investigating the role of p56lck in T-cell activation.
引用
收藏
页码：375 / 380
页数：6
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