CHARACTERIZATION OF A MONOCLONAL-ANTIBODY DIRECTED AGAINST THE PHOSPHOTYROSINE KINASE P56LCK, IN HUMAN T-CELLS

被引：10

作者：

ANSOTEGUI, IJ

CHOW, SC

JEDDITEHRANI, M

MELOCHE, S

PAWSON, A

SEKALY, RP

MAK, TW

WIGZELL, H

机构：

[1] ONTARIO CANC INST,TORONTO M4X 1K9,ONTARIO,CANADA

[2] INST RECH CLIN MONTREAL,IMMUNOL LAB,MONTREAL H2W 1R7,QUEBEC,CANADA

[3] SAMUEL LUNESFELD RES INST,TORONTO,ONTARIO,CANADA

来源：

SCANDINAVIAN JOURNAL OF IMMUNOLOGY | 1991年 / 33卷 / 04期

关键词：

D O I：

10.1111/j.1365-3083.1991.tb01784.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A monoclonal antibody (anti-p56lck) was generated against a fusion protein containing the residues 145-509 of the human p56lck, a lymphocyte-specific membrane-associated protein tyrosine kinase. The involvement of this enzyme in T-cell transmembrane signalling seems to be an early and crucial event during T-cell receptor-mediated activation. We have produced a monoclonal antibody which recognizes p56lck in free form and when associated with CD4. It functions in western blot analysis and is capable of selectively blocking auto-phosphorylation of this kinase. This monoclonal antibody should be useful for investigating the role of p56lck in T-cell activation.

引用

收藏

页码：375 / 380

页数：6

相关论文

共 27 条

[1] A FUNCTION FOR THE LCK PROTO-ONCOGENE [J].

BOLEN, JB ;

VEILLETTE, A .

TRENDS IN BIOCHEMICAL SCIENCES, 1989, 14 (10) :404-407

[2] PHOSPHORYLATION OF SYNTHETIC PEPTIDES BY A TYROSINE PROTEIN-KINASE FROM THE PARTICULATE FRACTION OF A LYMPHOMA CELL-LINE [J].

CASNELLIE, JE ;

HARRISON, ML ;

PIKE, LJ ;

HELLSTROM, KE ;

KREBS, EG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (02) :282-286

[3] PROTEIN IODINATION WITH SOLID-STATE LACTOPEROXIDASE [J].

DAVID, GS ;

REISFELD, RA .

BIOCHEMISTRY, 1974, 13 (05) :1014-1021

[4]

DFANIELIAN S, 1989, EUR J IMMUNOL, V19, P2183

[5] HIGH-LEVEL OF TYROSINE PROTEIN-KINASE IN A MURINE LYMPHOMA CELL-LINE INDUCED BY MOLONEY LEUKEMIA-VIRUS [J].

GACON, G ;

GISSELBRECHT, S ;

PIAU, JP ;

BOISSEL, JP ;

TOLLE, J ;

FISCHER, S .

EMBO JOURNAL, 1982, 1 (12) :1579-1582

[6] A NEW SURFACE-ANTIGEN ON HUMAN-LYMPHOCYTES DEFINED BY THE MURINE MONOCLONAL-ANTIBODY IVF7 [J].

GRUNEWALD, J ;

JANSON, CH ;

TEHRANI, MJ ;

MATSUO, Y ;

MELLSTEDT, H ;

WIGZELL, H .

SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 1990, 31 (04) :461-467

[7] A RAPID AND EFFICIENT IMMUNIZATION PROTOCOL FOR PRODUCTION OF MONOCLONAL-ANTIBODIES REACTIVE WITH AUTO-ANTIGENS [J].

HOLMDAHL, R ;

MORAN, T ;

ANDERSSON, M .

JOURNAL OF IMMUNOLOGICAL METHODS, 1985, 83 (02) :379-384

[8] ACTIVATORS OF PROTEIN KINASE-C INDUCE DISSOCIATION OF CD4, BUT NOT CD8, FROM P56LCK [J].

HURLEY, TR ;

LUO, KX ;

SEFTON, BM .

SCIENCE, 1989, 245 (4916) :407-409

[9] A HUMAN T-CELL-SPECIFIC CDNA CLONE (YT16) ENCODES A PROTEIN WITH EXTENSIVE HOMOLOGY TO A FAMILY OF PROTEIN-TYROSINE KINASES [J].

KOGA, Y ;

CACCIA, N ;

TOYONAGA, B ;

SPOLSKI, R ;

YANAGI, Y ;

YOSHIKAI, Y ;

MAK, TW .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1986, 16 (12) :1643-1646

[10]

KOGA Y, 1988, CANCER RES, V48, P856