IDENTIFICATION OF A 200-KDA GLYCOPROTEIN ANTIGEN OF SACCHAROMYCES-CEREVISIAE

被引：35

作者：

HEELAN, BT ^{[1
]}

ALLAN, S ^{[1
]}

BARNES, RMR ^{[1
]}

机构：

[1] UNIV LIVERPOOL,DEPT IMMUNOL,POB 147,LIVERPOOL L69 3BX,ENGLAND

来源：

IMMUNOLOGY LETTERS | 1991年 / 28卷 / 03期

关键词：

SACCHAROMYCES-CEREVISIAE; CROHNS DISEASE; GLYCOPROTEINS; ANTIBODIES; IMMUNOBLOTTING;

D O I：

10.1016/0165-2478(91)90001-Q

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

SDS-PAGE analysis of the soluble proteins of the yeast Saccharomyces cerevisiae revealed a single predominant moiety of apparent size 200 kDa which was strongly periodic acid Schiff-positive. In immunoblotting experiments, human sera containing anti-S. cerevisiae antibodies were reactive only with this glycoprotein (gp200); this was demonstrated using sera containing IgG and IgA isotype-specific anti-S. cerevisiae antibodies. The antigenicity of gp200 was substantially reduced by periodate treatment. These results show that gp200 is a major glycoprotein antigen of S. cerevisiae which is immunogenic in man.

引用

页码：181 / 186

页数：6

共 13 条

[1]

Ballou C, 1976, Adv Microb Physiol, V14, P93, DOI 10.1016/S0065-2911(08)60227-1

[2]

BALLOU CE, 1980, FUNGAL POLYSACCHARID, P1

[3] SERUM ANTIBODIES REACTIVE WITH SACCHAROMYCES-CEREVISIAE IN INFLAMMATORY BOWEL-DISEASE - IS IGA ANTIBODY A MARKER FOR CROHNS-DISEASE [J].

BARNES, RMR ;

ALLAN, S ;

TAYLORROBINSON, CH ;

FINN, R ;

JOHNSON, PM .

INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1990, 92 (01) :9-15

[4]

ESPITIA C, 1989, CLIN EXP IMMUNOL, V77, P378

[5] SACCHAROMYCES-CEREVISIAE STRUCTURAL CELL-WALL MANNOPROTEIN [J].

FREVERT, J ;

BALLOU, CE .

BIOCHEMISTRY, 1985, 24 (03) :753-759

[6]

JOHNSTONE A, 1987, IMMUNOCHEMISTRY PRAC

[7] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[8] ANTIBODY TO SELECTED STRAINS OF SACCHAROMYCES-CEREVISIAE (BAKERS AND BREWERS-YEAST) AND CANDIDA-ALBICANS IN CROHNS-DISEASE [J].

MCKENZIE, H ;

MAIN, J ;

PENNINGTON, CR ;

PARRATT, D .

GUT, 1990, 31 (05) :536-538

[9] EXPORT OF MAJOR CELL-SURFACE PROTEINS IS BLOCKED IN YEAST SECRETORY MUTANTS [J].

NOVICK, P ;

SCHEKMAN, R .

JOURNAL OF CELL BIOLOGY, 1983, 96 (02) :541-547

[10] GLYCOPROTEINS - WHAT ARE THE SUGAR CHAINS FOR [J].

PAULSON, JC .

TRENDS IN BIOCHEMICAL SCIENCES, 1989, 14 (07) :272-276

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