STUDY OF PHYSICOCHEMICAL AND THERMAL PROPERTIES OF L-PHENYLALANINE AMMONIA-LYASE

被引：1

作者：

Babich, O. O. ^{[1
]}

Ulrikh, E. V. ^{[2
]}

机构：

[1] Kemerovo Inst Food Sci & Technol, Bulv Stroitelei 47, Kemerovo 650056, Russia

[2] Kuzbass State Tech Univ, Kemerovo 650000, Russia

来源：

FOODS AND RAW MATERIALS | 2013年 / 1卷 / 02期

关键词：

L-phenylalanine ammonia-lyase; physicochemical and thermal properties; heating curves; cryoscopic temperature; lyophilization;

D O I：

10.12737/2056

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Physicochemical and thermal properties of L-phenylalanine ammonia-lyase preparation were studied. Thermal gravimetric analysis of physical and chemical phenomena occurring in the enzyme upon heating was conducted. Heating curves were registered. Kinetic parameters of the evaporation process were identified. Stability of the enzyme to freezing was verified. Cryoscopic temperature of a concentrated L-phenylalanine ammonia-lyase sample was identified. Chemical (dehydration, dissociation) and physical transformations accompanied by exothermic and endothermic effects were found to occur during freezing. Results of the thermal gravimetric analysis demonstrated feasibility of freeze-drying of L-phenylalanine ammonia-lyase preparation and allowed for determination of the optimum temperature ranges for thermal treatment and prediction of operational parameters of the drying process.

引用

页码：50 / 56

页数：7

共 10 条

[1] Effect of freeze drying and protectants on viability of the biocontrol yeast Candida sake [J].

Abadias, M ;

Benabarre, A ;

Teixidó, N ;

Usall, J ;

Viñas, I .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2001, 65 (03) :173-182

[2]

Babakin B. S., 2005, KHOLODILNAYA TEKHNIK, P56

[3]

BALDI G, 1994, EUR J PHARM BIOPHARM, V40, P138

[4] THE FREEZE-DRYING OF LACTIC-ACID BACTERIA - A REVIEW [J].

CHAMPAGNE, CP ;

GARDNER, N ;

BROCHU, E ;

BEAULIEU, Y .

CANADIAN INSTITUTE OF FOOD SCIENCE AND TECHNOLOGY JOURNAL-JOURNAL DE L INSTITUT CANADIEN DE SCIENCE ET TECHNOLOGIE ALIMENTAIRES, 1991, 24 (3-4) :118-128

[5]

Gusarov D. A., 2010, RUSS J BIOPHARMACEUT, V2, P3

[6]

Karpov A. M., 2000, SUSHKA PRODUKTOV MIK

[7]

Kretov I. T., 2001, KHRANENIE PERERABOTK, P12

[8]

Lykov A. V., 1968, TEORIYA SUCHKI

[9]

Nikitin E. E., 1971, ZAMORAZHIVANIE VYSUS

[10] Effect of carbohydrates on the survival of Lactobacillus helveticus during vacuum drying [J].

Santivarangkna, C ;

Kulozik, U ;

Foerst, P .

LETTERS IN APPLIED MICROBIOLOGY, 2006, 42 (03) :271-276

← 1 →