MINI-TN10 TRANSPOSON DERIVATIVES FOR INSERTION MUTAGENESIS AND GENE DELIVERY INTO THE CHROMOSOME OF GRAM-NEGATIVE BACTERIA

Four mini-Tn10 derivatives bearing the nptII, cat, aacC1 and tet genes along with MCS of the pBluescriptII plasmid were constructed. These derivatives are embedded into the gamma-ori R6K-based suicide plasmid pBSL177, which can be mobilized into a broad range of hosts by the RP4 plasmid, The relatively small size and the presence of the mutant ATS Tn2O transposase gene, fused to P-tac promoter, as well as the versatile MCS of the pBluescriptII plasmid make these vectors a reliable tool for insertion mutagenesis and chromosomal insertion of the cloned DNA fragments.