USE OF CRNA DIGOXIGENIN-LABELED PROBES FOR DETECTION OF ENTEROVIRUSES IN HUMANS AND IN THE ENVIRONMENT

被引：10

作者：

FUCHS, F

LEPARC, I

KOPECKA, H

GARIN, D

AYMARD, M

机构：

[1] INST PASTEUR,F-75724 PARIS 15,FRANCE

[2] HOP INSTRUCT ARMEES LYON,LYON,FRANCE

来源：

JOURNAL OF VIROLOGICAL METHODS | 1993年 / 42卷 / 2-3期

关键词：

ENTEROVIRUS; CRNA PROBE; MOLECULAR HYBRIDIZATION;

D O I：

10.1016/0166-0934(93)90034-O

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A dot blot hybridization test for enteroviruses is described using non-radioactive digoxigenin-labelled probes and a chemiluminescent detection. The use of a 5' non-coding riboprobe which detects all enteroviruses and a VP1 probe that detects the three serotypes of polioviruses allows the rapid detection of polioviruses and non-polio enteroviruses in human specimens or environmental water samples. The assay is strictly enterovirus specific and sensitive (800 fg RNA) and offers several advantages over conventional diagnosis or radioactive probes.

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收藏

页码：217 / 226

页数：10

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