KINETIC-STUDY ON STARCH HYDROLYSIS USING A GLUCOSE MALTOSE SENSING SYSTEM

被引：0
作者：
QU, HB
ZHANG, XE
CHUI, WW
ZHANG, SZ
LI, GX
FAN, OY
机构：
[1] CHINESE ACAD SCI,INST MICROBIOL,BEIJING 100080,PEOPLES R CHINA
[2] CHINESE ACAD SCI,INST CHEM MET,NATL LAB BIOCHEM ENGN,BEIJING 100080,PEOPLES R CHINA
来源：
BIOTECHNOLOGY TECHNIQUES | 1995年 / 9卷 / 06期
关键词：
D O I：
10.1007/BF00160834
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A dual-enzyme electrode flow injection system that can simultaneously determine glucose and maltose is used for an on-line study of starch hydrolyses catalysed by amylases. With the working system, determinations can be made every 2 minutes. A 10 mu L sample size with recycled back-flow minimises any loss of the reaction medium. The production, growth and decay of glucose and maltose concentrations during starch hydrolysis under various enzymatic conditions can thus be closely monitored, making it useful for the study of the catalytic kinetics of amylases and in screening and analysing enzyme systems.
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页码：445 / 450
页数：6
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