PEG1/MEST IMPRINTED GENE ON CHROMOSOME-6 IDENTIFIED BY CDNA SUBTRACTION HYBRIDIZATION

被引：242

作者：

KANEKOISHINO, T

KUROIWA, Y

MIYOSHI, N

KOHDA, T

SUZUKI, R

YOKOYAMA, M

VIVILLE, S

BARTON, SC

ISHINO, F

SURANI, MA

机构：

[1] TOKYO INST TECHNOL,CTR GENE RES,MIDORI KU,YOKOHAMA,KANAGAWA 226,JAPAN

[2] MITSUBISHI KASEI INST LIFE SCI,MACHIDA,TOKYO 194,JAPAN

[3] WELLCOME CRC INST CANC & DEV BIOL,CAMBRIDGE CB2 1QR,ENGLAND

[4] UNIV CAMBRIDGE,PHYSIOL LAB,CAMBRIDGE,ENGLAND

来源：

NATURE GENETICS | 1995年 / 11卷 / 01期

基金：

英国惠康基金;

关键词：

D O I：

10.1038/ng0995-52

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Parthenogenesis in the mouse is embryonic lethal partly because of imprinted genes that are expressed only from the paternal genome, In a systematic screen using subtraction hybridization between cDNAs from normal and parthenogenetic embryos, we initially identified two apparently novel imprinted genes, Peg1 and Peg3, Peg1 (paternally expressed gene 1) or Mest, the first imprinted gene found on the mouse chromosome 6, may contribute to the lethality of parthenogenones and of embryos with a maternal duplication for the proximal chromosome 6, Peg1/Mest is widely expressed in mesodermal tissues and belongs to the alpha/beta hydrolase fold family. A similar approach with androgenones can be used to identify imprinted genes that are expressed from the maternal genome only.

引用

页码：52 / 59

页数：8

共 38 条

[1] A NOVEL CDNA/PCR STRATEGY FOR EFFICIENT CLONING OF SMALL AMOUNTS OF UNDEFINED RNA [J].