A SIMPLE METHOD OF HLA-DRB TYPING USING ENZYMATICALLY AMPLIFIED DNA AND IMMOBILIZED PROBES ON MICROTITER PLATE

被引：31

作者：

KAWAI, S ^{[1
]}

MAEKAWAJIRI, S ^{[1
]}

TOKUNAGA, K ^{[1
]}

JUJI, T ^{[1
]}

YAMANE, A ^{[1
]}

机构：

[1] JAPANESE RED CROSS CENT BLOOD CTR,TOKYO,JAPAN

来源：

HUMAN IMMUNOLOGY | 1994年 / 41卷 / 02期

关键词：

D O I：

10.1016/0198-8859(94)90004-3

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We have developed a simple and economical method for HLA-DNA typing, called microtiter plate hybridization (PCR-MPH), which could replace standard PCR-SSO. This method is similar to that of an ELISA. Briefly, the PCR products labeled at the 5' termini with biotin were hybridized with probes immobilized on a microtiter well, and the bound PCR products were detected by streptavidin-conjugated enzymes followed by color development. A system for HLA-DRB1 ''generic'' typing (e.g., DR1, DR2), using microtiter wells coated with 12 different SSOs has been established. The HLA-DRB types classified using this method agreed well with those obtained by conventional serologic typing. The advantages of this microtiter plate-hybridization method for routine HLA-DNA typing are a short assay time, easy processing of large numbers of samples, and the potential for automation.

引用

页码：121 / 126

页数：6

共 20 条

[1]

BANNAI M, 1994, IN PRESS EUR J IMMUN

[2] NOMENCLATURE FOR FACTORS OF THE HLA SYSTEM, 1991 [J].