UBIQUITIN POOLS, UBIQUITIN MESSENGER-RNA LEVELS, AND UBIQUITIN-MEDIATED PROTEOLYSIS IN AGING HUMAN FIBROBLASTS

被引：28

作者：

PAN, JX ^{[1
]}

SHORT, SR ^{[1
]}

GOFF, SA ^{[1
]}

DICE, JF ^{[1
]}

机构：

[1] TUFTS UNIV,SCH MED,DEPT PHYSIOL,136 HARRISON AVE,BOSTON,MA 02111

来源：

EXPERIMENTAL GERONTOLOGY | 1993年 / 28卷 / 01期

关键词：

D O I：

10.1016/0531-5565(93)90018-9

中图分类号：

R592 [老年病学]; C [社会科学总论];

学科分类号：

03 ; 0303 ; 100203 ;

摘要：

Senescent cells have less free ubiquitin and more ubiquitin-protein conjugates than do young cells. The ubiquitin-protein conjugates are heterogeneous in size but contain prominent bands at 106, 55, and 22 kDa. The age-related increase in ubiquitin-protein conjugates applies primarily to the 55-kDa band, while the 106-kDa and 22-kDa conjugates change little with age. Ubiquitin mRNA levels do not change with age, and the ability of cells to degrade two proteins that are good substrates for ubiquitin-mediated proteolysis is unaltered by aging. These results indicate that an increase in ubiquitin-protein conjugates does not necessarily reflect alterations in ubiquitin-mediated proteolysis. Furthermore, an overactive pathway of ubiquitin-mediated proteolysis does not appear to contribute to the proliferative arrest in senescent cells.

引用

页码：39 / 49

页数：11

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