HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF URINARY OLIGOSACCHARIDES IN THE DIAGNOSIS OF GLYCOPROTEIN DEGRADATION DISORDERS

被引：11

作者：

HOMMES, FA

VARGHESE, M

机构：

[1] Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta

来源：

CLINICA CHIMICA ACTA | 1991年 / 203卷 / 2-3期

关键词：

HPLC; URINARY OLIGOSACCHARIDES; GLYCOPROTEIN DEGRADATION;

D O I：

10.1016/0009-8981(91)90293-L

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Urinary oligosaccharides can be separated by high-performance anion-exchange chromatography using a Dionex CarboPac PA1 column, elution with aqueous sodium hydroxide and sodium acetate solutions and detection by pulsed amperometry. Each of the urines of patients with glycoprotein degradation disorders yielded a pattern of oligosaccharide excretion unique for that disorder, facilitating an unambiguous diagnosis. The method is sensitive (10-mu-l of urine required) and fast (40 min).

引用

页码：211 / 224

页数：14

共 13 条

[1]

BLOM W, 1983, CLIN CHIM ACTA, V134, P224

[2]

CAPPA GV, 1990, LANCET, V335, P569

[3] OLIGOSACCHARIDES IN URINE OF PATIENTS WITH GLYCOPROTEIN STORAGE DISEASES .1. RAPID DETECTION BY THIN-LAYER CHROMATOGRAPHY [J].