MATRIX EFFECTS IN IMMUNOASSAYS

被引：69
作者：
WOOD, WG
机构：
[1] Clinical Laboratories, Dept. of Internal Medicine, Medical University of Liibeck, DE, W-2400
来源：
SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION | 1991年 / 51卷
关键词：
D O I：
10.3109/00365519109104608
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
This article deals with "matrix effects" in immunoassay systems. As each component has its own matrix (buffer, antibody, separation agents), the term matrix effect - usually only referring to the matrix of the sample - has been replaced by the term interference effect. The possible sites of interference have been dealt with in turn, taking each component/step of an immunoassay separately. Results of assay improvement due to the removal of "matrix effects" are given using the PTH-assay as example. The change in results due to tampering with the serum matrix is given using the determination of free thyroxine in commercial quality control sera.
引用
收藏
页码：105 / 112
页数：8
相关论文
共 54 条
[1]  
BENITEZ BL, 1975, ARCH INVEST MED MEX, V6, P55
[2]   INTERFERENCE OF COMPLEMENT WITH THE BINDING OF CARCINOEMBRYONIC ANTIGEN TO SOLID-PHASE MONOCLONAL-ANTIBODIES [J].
BORMER, OP .
JOURNAL OF IMMUNOLOGICAL METHODS, 1989, 121 (01) :85-93
[3]   SPECIFICITY AND SENSITIVITY OF THE IGM CAPTURE IMMUNOASSAY - STUDIES OF POSSIBLE FACTORS INDUCING FALSE POSITIVE OR FALSE NEGATIVE RESULTS [J].
BRIANTAIS, MJ ;
GRANGEOTKEROS, L ;
PILLOT, J .
JOURNAL OF VIROLOGICAL METHODS, 1984, 9 (01) :15-26
[4]   SOLID-PHASE RADIOIMMUNOASSAY IN ANTIBODY-COATED TUBES [J].
CATT, K ;
TREGEAR, GW .
SCIENCE, 1967, 158 (3808) :1570-&
[5]   REDUCTION OF INTERFERENCE BY SPECIFIC IGG WITH A MODIFIED MICROTITER SOLID-PHASE RADIOIMMUNOASSAY TO MEASURE HONEYBEE VENOM IGE [J].
CHEUNG, NKV ;
BLESSINGMOORE, J ;
REID, MJ ;
LEWISTON, NJ ;
YANG, G .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1983, 71 (03) :283-293
[6]  
COBBLEDICK RE, 1984, CLIN CHEM, V30, P1264
[7]   C-TERMINAL AND N-TERMINAL SPECIFIC LH-RELEASING HORMONE (LRH) RADIOIMMUNOASSAY [J].
DAHLEN, HG ;
VOIGT, KH ;
SCHNEIDER, HPG .
HORMONE AND METABOLIC RESEARCH, 1976, 8 (01) :61-66
[8]   SPIRONOLACTONE INTERFERENCE WITH DIGOXIN RADIOIMMUNOASSAY IN CIRRHOTIC-PATIENTS [J].
DIPIRO, JT ;
COTE, JR ;
DIPIRO, CR ;
BUSTRACK, JA .
AMERICAN JOURNAL OF HOSPITAL PHARMACY, 1980, 37 (11) :1518-1521
[9]   BIOCHEMICAL-STUDIES IN PERITONEAL-FLUID FROM PATIENTS WITH ACUTE-PANCREATITIS - RELATIONSHIP TO ETIOLOGY [J].
DUBICK, MA ;
MAYER, AD ;
MAJUMDAR, APN ;
MAR, G ;
MCMAHON, MJ ;
GEOKAS, MC .
DIGESTIVE DISEASES AND SCIENCES, 1987, 32 (03) :305-312
[10]   COMPARISON OF DIFFERENT SANDWICH ENZYME IMMUNOASSAYS FOR THE QUANTITATION OF HUMAN APOLIPOPROTEIN-A-I AND APOLIPOPROTEIN-A-II [J].
DUBOIS, DY ;
CANTRAINE, F ;
MALMENDIER, CL .
JOURNAL OF IMMUNOLOGICAL METHODS, 1987, 96 (01) :115-120
123456