USE OF ELECTROSPRAY MASS-SPECTROMETRY TO ASSAY CHEMICAL-REACTIVITY OF AN ACYL CARRIER PROTEIN INVOLVED IN FATTY-ACID BIOSYNTHESIS IN SACCHAROPOLYSPORA-ERYTHRAEA

被引：14

作者：

BRIDGES, AM ^{[1
]}

LEADLAY, PF ^{[1
]}

REVILL, WP ^{[1
]}

STAUNTON, J ^{[1
]}

机构：

[1] CAMBRIDGE CTR MOLEC RECOGNIT,DEPT BIOCHEM,CAMBRIDGE CB2 1QW,ENGLAND

来源：

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS | 1991年 / 11期

关键词：

D O I：

10.1039/c39910000776

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Electrospray mass spectrometry (ESMS) is used to monitor the chemical reactivity of the thiol-containing 4'-phosphopantetheine residue of an acyl carrier protein implicated in fatty acid biosynthesis in Saccharopolyspora erythraea.

引用

页码：776 / 777

页数：2

共 7 条

[1] COMPONENT FATTY ACIDS OF LIPIDS FROM SOME STREPTOMYCES SPP [J].

BALLIO, A ;

BARCELLONA, S ;

BONIFORTI, L .

BIOCHEMICAL JOURNAL, 1965, 94 (01) :C11-+

[2] CHEMICAL SYNTHESIS OF ACYL THIOESTERS OF ACYL CARRIER PROTEIN WITH NATIVE STRUCTURE [J].

CRONAN, JE ;

KLAGES, AL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (09) :5440-5444

[3] ELECTROSPRAY IONIZATION FOR MASS-SPECTROMETRY OF LARGE BIOMOLECULES [J].

FENN, JB ;

MANN, M ;

MENG, CK ;

WONG, SF ;

WHITEHOUSE, CM .

SCIENCE, 1989, 246 (4926) :64-71

[4] A SMALL, DISCRETE ACYL CARRIER PROTEIN IS INVOLVED IN DE NOVO FATTY-ACID BIOSYNTHESIS IN STREPTOMYCES-ERYTHRAEUS [J].

HALE, RS ;

JORDAN, KN ;

LEADLAY, PF .

FEBS LETTERS, 1987, 224 (01) :133-136

[5]

NUMA S, 1984, COMPREHENSIVE BIOCH, V7

[6]

REVILL WP, 1991, IN PRESS J BACTERIOL

[7] GENETICS AND REGULATION OF BACTERIAL LIPID-METABOLISM [J].

VANDENBOOM, T ;

CRONAN, JE .

ANNUAL REVIEW OF MICROBIOLOGY, 1989, 43 :317-343

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