ISOLATION AND CHARACTERIZATION OF DNA-SEQUENCES FROM FLOW-SORTED HUMAN-CHROMOSOME 22 LIBRARIES

被引：5

作者：

METZDORF, R ^{[1
]}

HERZOG, R ^{[1
]}

GOTTERT, E ^{[1
]}

BLIN, N ^{[1
]}

机构：

[1] UNIV SAARLAND,DEPT HUMAN GENET,W-6650 HOMBURG,GERMANY

来源：

HUMAN HEREDITY | 1990年 / 40卷 / 03期

关键词：

chromosome; 22; DNA libraries; DNA polymorphism; mapping;

D O I：

10.1159/000153922

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Two flow-sorted chromosome 22 libraries were used to isolate DNA sequences specific for chromosome 22. 45-phage DNAs were probed against human genomic DNA. 12 of them showed unique or low-copycharacter. Using digested DNA from rodent-human hybrid cell lines, 3 of the 12 recombinants were assigned unique to chromosome 22 and regionally mapped. 1 clone mapped to 22pter-q11, 1 clone to 22q12-qter and 1 clone, for which in situ hybridization was performed, to 22q13.1. 2 low-copy probes, 1 of them displaying polymorphisms in MspI and TaqI digests of individual DNAs, must have similar sequences on 22 and additional chromosomes. Furthermore, a highly repetitive DNA representing a compound locus of some hundred kilobases on chromosome 22 was isolated. These 6 probes may provide useful tools for studying the structure and function of this small chromosome involved in a relatively high number of inherited and acquired diseases.

引用

页码：145 / 152

页数：8

共 50 条

[1] ISOLATION OF DNA-SEQUENCES ON HUMAN CHROMOSOME-21 BY APPLICATION OF RECOMBINATION-BASED ASSAY TO DNA FROM FLOW-SORTED CHROMOSOMES
TANTRAVAHI, U
MCNEIL, G
STEWART, GD
ROY, S
PATTERSON, D
VANKEUREN, M
DRABKIN, HA
LALANDE, M
KURNIT, DM
LATT, SA
CYTOGENETICS AND CELL GENETICS, 1987, 46 (1-4): : 703 - 703
[2] ISOLATION OF DNA-SEQUENCES ON HUMAN CHROMOSOME-21 BY APPLICATION OF A RECOMBINATION-BASED ASSAY TO DNA FROM FLOW-SORTED CHROMOSOMES
TANTRAVAHI, U
STEWART, GD
VANKEUREN, M
MCNEIL, G
ROY, S
PATTERSON, D
DRABKIN, H
LALANDE, M
KURNIT, DM
LATT, SA
HUMAN GENETICS, 1988, 79 (03) : 196 - 202
[3] REGIONAL MAPPING OF UNIQUE DNA-SEQUENCES FROM HUMAN CHROMOSOME-3 DERIVED FROM A FLOW-SORTED CHROMOSOME LIBRARY
ATCHISON, LM
NAYLOR, SL
FREED, JJ
COMIS, RL
CYTOGENETICS AND CELL GENETICS, 1988, 48 (03): : 156 - 159
[4] ISOLATION OF HUMAN-CHROMOSOME 13-SPECIFIC DNA-SEQUENCES CLONED FROM FLOW SORTED CHROMOSOMES AND POTENTIALLY LINKED TO THE RETINOBLASTOMA LOCUS
LALANDE, M
DRYJA, TP
SCHRECK, RR
SHIPLEY, J
FLINT, A
LATT, SA
CANCER GENETICS AND CYTOGENETICS, 1984, 13 (04) : 283 - 295
[5] CONSTRUCTION OF HUMAN CHROMOSOME-SPECIFIC DNA LIBRARIES FROM FLOW-SORTED CHROMOSOMES
DEAVEN, LL
VANDILLA, MA
BARTHOLDI, MF
CARRANO, AV
CRAM, LS
FUSCOE, JC
GRAY, JW
HILDEBRAND, CE
MOYZIS, RK
PERLMAN, J
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1986, 51 : 159 - 167
[6] ISOLATION OF HUMAN-CHROMOSOME 21-SPECIFIC DNA-SEQUENCES
KORENBERG, JR
CROYLE, ML
COX, DR
CYTOGENETICS AND CELL GENETICS, 1985, 40 (1-4): : 673 - 673
[7] CONSTRUCTION AND CHARACTERIZATION OF PARTIAL DIGEST DNA LIBRARIES MADE FROM FLOW-SORTED HUMAN CHROMOSOME-16
LONGMIRE, JL
BROWN, NC
MEINCKE, LJ
CAMPBELL, ML
ALBRIGHT, KL
FAWCETT, JJ
CAMPBELL, EW
MOYZIS, RK
HILDEBRAND, CE
EVANS, GA
DEAVEN, LL
GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING, 1993, 10 (3-4): : 69 - 76
[8] ISOLATION AND REGIONAL MAPPING OF DNA-SEQUENCES UNIQUE TO HUMAN-CHROMOSOME 21
KORENBERG, JR
CROYLE, ML
COX, DR
AMERICAN JOURNAL OF HUMAN GENETICS, 1987, 41 (06) : 963 - 978
[9] ISOLATION OF ANONYMOUS DNA MARKERS FOR HUMAN-CHROMOSOME 22Q11 FROM A FLOW-SORTED LIBRARY, AND MAPPING USING HYBRIDS FROM PATIENTS WITH DIGEORGE SYNDROME
SHARKEY, AM
MCLAREN, L
CARROLL, M
FANTES, J
GREEN, D
WILSON, D
SCAMBLER, PJ
EVANS, HJ
HUMAN GENETICS, 1992, 89 (01) : 73 - 78
[10] ISOLATION OF GIANT DNA FRAGMENTS FROM FLOW-SORTED HUMAN-CHROMOSOMES
MINOSHIMA, S
KAWASAKI, K
FUKUYAMA, R
MAEKAWA, M
KUDOH, J
SHIMIZU, N
CYTOMETRY, 1990, 11 (04): : 539 - 546

← 1 2 3 4 5 →