AUTOREGULATION OF ACTIN SYNTHESIS IN HEPATOCYTES BY TRANSCRIPTIONAL AND POSTTRANSCRIPTIONAL MECHANISMS

被引:30
作者
REUNER, KH
WIEDERHOLD, M
DUNKER, P
JUST, I
BOHLE, RM
KROGER, M
KATZ, N
机构
[1] UNIV SAARLAND,INST PHARMAKOL,HOMBURG,GERMANY
[2] UNIV GIESSEN,INST PATHOL,GIESSEN,GERMANY
[3] UNIV GIESSEN,INST MIKROBIOL & MOLEK BIOL,GIESSEN,GERMANY
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1995年 / 230卷 / 01期
关键词
ACTIN; BOTULINUM C2 TOXIN; PHALLOIDIN; HEPATOCYTE;
D O I
10.1111/j.1432-1033.1995.0032i.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Treatment of rat hepatocytes with the filamentous-actin-stabilizing toxin phalloidin decreased the amount of globular actin by 77% in the cytosol and by 80% in the nucleus within 12 h. Simultaneously, actin mRNA was specifically increased by 230%. The de-novo synthesis of actin mRNA, as measured by nuclear run-on transcription, was enhanced by 250%. Treatment of cells with actinomycin D blocked the increase of actin mRNA. The apparent half-life of actin mRNA was not significantly altered during treatment with phalloidin. In contrast, the globular-actin-stabilizing botulinum C2 toxin increased the amount of cytosolic globular actin by 50% within 12 h. Simultaneously, the actin mRNA level was decreased by 62%. However, de-novo synthesis of actin mRNA was not impaired. The apparent half-life of actin mRNA was decreased by approximately 60% during treatment with C2 toxin. The data strongly suggest an autoregulatory control of actin synthesis on the basis of the globular/filamentous actin ratio in rat hepatocytes at the transcriptional as well as at the posttranscriptional levels.
引用
收藏
页码:32 / 37
页数:6
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