APPLICATIONS OF THIN-LAYER CHROMATOGRAPHY, HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND MASS-SPECTROMETRY IN THE FERMENTATION AND ISOLATION OF THE ANTIBIOTIC NYBOMYCIN

被引：5

作者：

POPE, JA ^{[1
]}

NELSON, RA ^{[1
]}

SCHAFFNER, CP ^{[1
]}

ROSEN, RT ^{[1
]}

PANDEY, RC ^{[1
]}

机构：

[1] RUTGERS STATE UNIV,WAKSMAN INST MICROBIOL,PISCATAWAY,NJ 08854

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY | 1990年 / 6卷 / 01期

关键词：

Deoxynybomycin; Fermentation; High performance liquid chromatography; Mass spectrometry; Nybomycin; Nybomycin acetate; Thin layer chromatography;

D O I：

10.1007/BF01576178

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and mass spectrometry (MS) methods have been developed for the analysis of the antibiotic nybomycin, its derivatives deoxynybomycin and nybomycin acetate, during the fermentation and isolation of nybomycin. Using a quantitative HPLC based assay, the time course of nybomycin production (nybomycin titers) in 1000 liter fermentations was determined. Desorption chemical ionization mass spectrometry (DCI/MS) of standard nybomycin samples, fermentation broth samples and purified fractions suggested the co-production of deoxynybomycin which was not reported previously from this organism. TLC and HPLC were used to confirm the presence of deoxynybomycin in the crude extracts of fermentation broths. © 1990 Society for Industrial Microbiology.

引用

页码：61 / 69

页数：9

共 9 条

[1]

Brock T.D., Sokolski W.T., Biological studies on the antibiotic nybomycin, Antibiot. Chemother., 8, pp. 631-636, (1958)

[2]

Eble T.E., Boyack G.A., Large C.M., DeVries W.H., Nybomycin: Isolation, properties and derivatives, Antibiot. Chemother., 8, pp. 627-630, (1958)

[3]

Knoll W.M.J., Huxtable R.J., Rinehart K.L., Nybomycin. VI. Incorporation of acetate-13C, acetate-14C, and methionine-14C, Journal of the American Chemical Society, 95, pp. 2703-2705, (1973)

[4]

Nadzan A.M., Rinehart K.L., Nybomycin. 8. Biosynthetic origin of the central ring carbons studied by13C-labeled substrates, J. Am. Chem. Soc., 98, pp. 5012-5014, (1976)

[5]

Nadzan A.M., Rinehart K.L., Nybomycin. 9. Synthetic and biosynthetic incorporation of15N as a means of assigning the13C nuclear magnetic resonance spectrum of nybomycin, J. Am. Chem. Soc., 99, pp. 4647-4654, (1977)

[6]

Naganawa H., Wakashiro T., Yagi A., Kondo S., Takita T., Hamada M., Maeda K., Umezawa H., Deoxynybomycin from a Streptomyces, J. Antibiot., 23, pp. 365-368, (1970)

[7]

Rinehart K.L., Renfroe H.B., The structure of nybomycin, J. Am. Chem. Soc., 83, pp. 3729-3730, (1961)

[8]

Strelitz F., Flon H., Asheshov I.N., Nybomycin, a new antibiotic with antiphage and antibacterial properties, Proceedings of the National Academy of Sciences, 41, pp. 620-624, (1955)

[9]

Umezawa I., Takano S., A new antitumor antibiotic, kazusamycin, J. Antibiot., 37, pp. 706-711, (1984)

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