THE ROLE OF mutR GENE IN METABOLISM AND VIRULENCE OF emm12 GENOTYPE STREPTOCOCCUS PYOGENES STRAINS

被引：1

作者：

Zutkis, A. A. ^{[1
]}

Milman, B. L. ^{[2
]}

Dmitriev, A. V. ^{[3
,4
]}

机构：

[1] Inst Expt Med, Dept Mol Microbiol, Lab Funct Genom & Prote Microorganisms, St Petersburg, Russia

[2] Inst Expt Med, Dept Mol Microbiol, Lab Biomed & Pharmaceut Mass Spectrometry, St Petersburg, Russia

[3] Inst Expt Med, Sci, St Petersburg, Russia

[4] Inst Expt Med, Dept Ecol Physiol, St Petersburg, Russia

来源：

INFEKTSIYA I IMMUNITET | 2014年 / 4卷 / 04期

关键词：

Streptococcus pyogenes; mutR; transcriptional regulation; metabolism; virulence;

D O I：

10.15789/2220-7619-2014-4-339-346

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

In the present study the functional role of the mutR regulatory protein gene of Streptococcus pyogenes (emm12) was studied. The mutR gene was inactivated in the strains no. 97 and no. 152 by insertional mutagenesis. Inactivation of the mutR gene was found to affect the dynamic and characteristics of bacterial growth in liquid medium. Expression of secreted nucleases was significantly lower in the mutant strains compared to the wild-type strains. Two-dimensional electrophoresis and mass-spectrometry revealed differences in expression of number of the proteins in mutant strains compared to the wild-type strains. Inactivation of the mutR gene negatively affected capacity of S. pyogenes to adhere to human epithelial cells. Finally, the virulence properties of the no. 152[mutR] mutant strains were found to be 4,7-fold less compared to the strain no. 152, while the no. 97[mutR] mutant strain became avirulent compared to the strain no. 97 due to insertional inactivation of the mutR gene.

引用

页码：339 / 346

页数：8

共 27 条

[1] Identification of Rgg Binding Sites in the Streptococcus pyogenes Chromosome [J].

Anbalagan, Srivishnupriya ;

McShan, W. Michael ;

Dunman, Paul M. ;

Chaussee, Michael S. .

JOURNAL OF BACTERIOLOGY, 2011, 193 (18) :4933-4942

[2] Group A streptococcal rofA gene is involved in the control of several virulence genes and eukaryotic cell attachment and internalization [J].

Beckert, S ;

Kreikemeyer, B ;

Podbielski, A .

INFECTION AND IMMUNITY, 2001, 69 (01) :534-537

[3] Two Group A Streptococcal Peptide Pheromones Act through Opposing Rgg Regulators to Control Biofilm Development [J].

Chang, Jennifer C. ;

LaSarre, Breah ;

Jimenez, Juan C. ;

Aggarwal, Chaitanya ;

Federle, Michael J. .

PLOS PATHOGENS, 2011, 7 (08)

[4] Rgg regulates growth phase-dependent expression of proteins associated with secondary metabolism and stress in Streptococcus pyogenes [J].

Chaussee, MA ;

Callegari, EA ;

Chaussee, MS .

JOURNAL OF BACTERIOLOGY, 2004, 186 (21) :7091-7099

[5] Rgg coordinates virulence factor synthesis and metabolism in Streptococcus pyogenes [J].

Chaussee, MS ;

Somerville, GA ;

Reitzer, L ;

Musser, JM .

JOURNAL OF BACTERIOLOGY, 2003, 185 (20) :6016-6024

[6] Srv Mediated Dispersal of Streptococcal Biofilms Through SpeB Is Observed in CovRS plus Strains [J].

Connolly, Kristie L. ;

Braden, Amy K. ;

Holder, Robert C. ;

Reid, Sean D. .

PLOS ONE, 2011, 6 (12)

[7] Pathogenesis of group A streptococcal infections [J].

Cunningham, MW .

CLINICAL MICROBIOLOGY REVIEWS, 2000, 13 (03) :470-+

[8] Streptococcus pyogenes Arginine and Citrulline Catabolism Promotes Infection and Modulates Innate Immunity [J].

Cusumano, Zachary T. ;

Watson, Michael E., Jr. ;

Caparon, Michael G. .

INFECTION AND IMMUNITY, 2014, 82 (01) :233-242

[9]

[ДМИТРИЕВ А.В. Dmitriev A.V.], 2009, [Медицинский академический журнал, Meditsinskii akademicheskii zhurnal], V9, P50

[10] Inter- and intraserotypic variation in the Streptococcus pyogenes Rgg regulon [J].

Dmitriev, Alexander V. ;

McDowell, Emily J. ;

Chaussee, Michael S. .

FEMS MICROBIOLOGY LETTERS, 2008, 284 (01) :43-51

← 1 2 3 →