HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 (HIV-1) TAT-PROTEIN STIMULATES THE PRODUCTION OF INTERLEUKIN-6 (IL-6) BY PERIPHERAL-BLOOD MONOCYTES

被引：0
作者：
ZAULI, G
FURLINI, G
RE, MC
MILANI, D
CAPITANI, S
LAPLACA, M
机构：
来源：
MICROBIOLOGICA | 1993年 / 16卷 / 02期
关键词：
TAT-PROTEIN; HIV-1; IL-6; GM-CSF; TNF-ALPHA;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
In this study we evaluated the effect of human immunodeficiency virus type 1 (HIV-1) recombinant tat-protein on the production of interleukin-6 (IL-6), granulocyte/macrophage colony stimulating factor (GM-CSF) and tumor necrosis factor-alpha (TNF-alpha) by purified peripheral blood monocytes. Whereas no effects were observed on TNF-alpha and GM-CSF production, recombinant tat-protein was able to induce the production of IL-6 by peripheral blood monocytes in a dose-dependent fashion for concentrations ranging from 1 ng/ml to 1 mug/ml. Pre-exposure of tat-protein with a polyclonal neutralizing anti-tat antibody (dilution 1:100) completely abrogated the tat-dependent increase in IL-6 production. The ability of tat-protein to selectively stimulate the production of IL-6 by peripheral blood monocytes could help to explain the presence of elevated levels of IL-6 in the serum of HIV-1 seropositive individuals, especially in patients in advanced stages of the disease with an active viral replication.
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页码：115 / 120
页数：6
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共 47 条
[41]   Hepatitis C virus core protein enhances HIV-1 replication in human macrophages through TLR2, JNK, and MEK1/2-dependent of TNF-α and IL-6 [J].
Swaminathan, Gokul ;
Pascual, Daniel ;
Rival, Germaine ;
Perales-Linares, Renzo ;
Martin-Garcia, Julio ;
Navas-Martin, Sonia .
FEBS LETTERS, 2014, 588 (18) :3501-3510
[42]   Pathogenic prion protein fragment (PrP106-126) promotes human immunodeficiency virus type-1 infection in peripheral blood monocyte-derived macrophages [J].
Bacot, Silvia M. ;
Feldman, Gerald M. ;
Yamada, Kenneth M. ;
Dhawan, Subhash .
VIROLOGY, 2015, 476 :372-376
[43]   HIGH-AFFINITY BINDING OF TAR RNA BY THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 TAT PROTEIN REQUIRES BASE-PAIRS IN THE RNA STEM AND AMINO-ACID-RESIDUES FLANKING THE BASIC REGION [J].
CHURCHER, MJ ;
LAMONT, C ;
HAMY, F ;
DINGWALL, C ;
GREEN, SM ;
LOWE, AD ;
BUTLER, PJG ;
GAIT, MJ ;
KARN, J .
JOURNAL OF MOLECULAR BIOLOGY, 1993, 230 (01) :90-110
[44]   In vitro TNF-α and IL-6 production by adherent peripheral blood mononuclear cells obtained from type 1 and type 2 diabetic patients evaluated according to the metabolic control [J].
Foss-Freitas, Maria Cristina ;
Foss, Norma Tiraboschi ;
Donadi, Eduardo Antonio ;
Foss, Milton Cesar .
IMMUNOLOGY OF DIABETES IV: PROGRESS IN OUR UNDERSTANDING, 2006, 1079 :177-180
[45]   PROGRESSION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 (HIV-1) INFECTION AMONG HOMOSEXUAL MEN IN HEPATITIS-B VACCINE TRIAL COHORTS IN AMSTERDAM, NEW-YORK-CITY, AND SAN-FRANCISCO, 1978-1991 [J].
HESSOL, NA ;
KOBLIN, BA ;
VANGRIENSVEN, GJP ;
BACCHETTI, P ;
LIU, JY ;
STEVENS, CE ;
COUTINHO, RA ;
BUCHBINDER, SP ;
KATZ, MH .
AMERICAN JOURNAL OF EPIDEMIOLOGY, 1994, 139 (11) :1077-1087
[46]   Interleukin-6 release by cultured peripheral blood mononuclear cells inversely correlates with height velocity, bone age, insulin-like growth factor-I, and insulin-like growth factor binding protein-3 serum levels in children with perinatal HIV-1 infection [J].
de Martino, M ;
Galli, L ;
Chiarelli, F ;
Verrotti, A ;
Rossi, ME ;
Bindi, G ;
Galluzzi, F ;
Salti, R ;
Vierucci, A .
CLINICAL IMMUNOLOGY, 2000, 94 (03) :212-218
[47]   Retention of marked sensitivity to (S)-4-isopropoxycarbonyl-6-methoxy-3-(methylthiomethyl)-3,4-dihydroquinoxaline-2(1H)-thione (HBY 097) by an azidothymidine (AZT)-resistant human immunodeficiency virus type 1 (HIV-1) strain subcultured in the combined presence of quinoxaline HBY 097 and 2′,3′-dideoxy-3′-thiacytidine (lamivudine) [J].
Balzarini, J ;
Pelemans, H ;
Riess, G ;
Roesner, M ;
Winkler, I ;
De Clercq, E ;
Kleim, JP .
BIOCHEMICAL PHARMACOLOGY, 1998, 55 (05) :617-625
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